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SW480 [SW-480] (人結(jié)腸癌細(xì)胞) (STR鑒定正確)
貨號(hào)：CL-0223
規(guī)格：1×10?cells/T25培養(yǎng)瓶
價(jià)格： ￥1600
生長(zhǎng)培養(yǎng)基：Leibovitz's L-15＋10% FBS＋1% P/S
產(chǎn)品概述
| 名稱(chēng) | SW480 [SW-480] (人結(jié)腸癌細(xì)胞) (STR鑒定正確) |
| 別稱(chēng) | SW-480; SW 480; SW480E |
| 種屬 | 人類(lèi) |
| 生長(zhǎng)特性 | 貼壁細(xì)胞 |
| 細(xì)胞形態(tài) | 上皮細(xì)胞樣 |
| 凍存條件 | 凍存液：55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度：液氮 |
| 培養(yǎng)方案A（默認(rèn)） |
生長(zhǎng)培養(yǎng)基:
培養(yǎng)條件:
氣相：空氣，100%, 溫度：37℃
|
| 培養(yǎng)方案B（可選） |
培養(yǎng)條件:
氣相：空氣，95%；CO2，5%, 溫度：37℃
|
| 推薦傳代比例 | 1:2-1:4 |
| 推薦換液頻率 | 2-3次/周 |
| 注意事項(xiàng) | 1. 該細(xì)胞推薦使用Leibovitz's L-15培養(yǎng)基進(jìn)行培養(yǎng)，Leibovitz's L-15不可以通入二氧化碳，會(huì)產(chǎn)生細(xì)胞毒性； 2. 該細(xì)胞不建議更換為DMEM培養(yǎng)，圓形較多，效果不好。 |
| 背景描述 | SW480 [SW-480]細(xì)胞源自原位直腸腺癌，和SW620細(xì)胞源自同一病人一年后的淋巴結(jié)轉(zhuǎn)移。CSAp和直腸抗體3陰性；角蛋白免疫過(guò)氧化物酶染色陽(yáng)性。p53基因第273位密碼子的G→A突變引起Arg→His替代，309位密碼子的C→T突變導(dǎo)致Pro→Ser替代。細(xì)胞p53蛋白表達(dá)水平提高，癌基因c-myc、K-ras、H-ras、N-ras、myb、sis和fos的表達(dá)呈陽(yáng)性，癌基因N-myc的表達(dá)未做檢測(cè)。SW480 [SW-480]細(xì)胞不表達(dá)細(xì)胞溶解酶，一種與腫瘤入侵相關(guān)的金屬蛋白酶。有報(bào)道稱(chēng)，SW480 [SW-480]細(xì)胞表達(dá)GM-CSF。SW480 [SW-480]細(xì)胞ras原癌基因的12位密碼子有一個(gè)突變，可以用作PCR法檢測(cè)該突變的陽(yáng)性對(duì)照。1978年11月，A·Leibovitz將其提交給ATCC時(shí)已傳代至第91代。 |
| 年齡（性別） | 男性；50歲 |
| 組織來(lái)源 | 直腸；結(jié)直腸腺癌 |
| 細(xì)胞類(lèi)型 | 腫瘤細(xì)胞 |
| 腫瘤類(lèi)型 | 腸癌細(xì)胞 |
| 生物安全等級(jí) | BSL-1 |
| 倍增時(shí)間 | ~30-50 hours |
| 致瘤性 | Yes. Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10^7 cells. |
| 受體表達(dá)情況 | epidermal growth factor (EGF) |
| 基因表達(dá)情況 | carcinoembryonic antigen (CEA) 0.7 ng/10^6 cells/10 days; keratin; transforming growth factor beta, myc+; myb+ ; ras+; fos+; sis+; p53+; abl -; ros -; src -, HLA A2, B8, B17; Blood Type A; Rh+, The cells are positive for keratin by immunoperoxidase staining., The line is positive for expression of c-myc, K-ras, H-ras, N-ras, myb, sis and fos oncogenes. |
| 保藏機(jī)構(gòu) | ATCC; CCL-228 DSMZ; ACC-313 ECACC; 87092801 |
STR鑒定
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STR位點(diǎn)信息
Amelogenin X CSF1PO 13,14 D2S1338 17,24 D3S1358 15 D5S818 13 D7S820 8 D8S1179 13 D13S317 12 D16S539 13 D18S51 13 D19S433 13 D21S11 30,30.2 FGA 24 PentaD 9,15 PentaE 10 TH01 8 TPOX 11 vWA 16 D6S1043 11,12 D12S391 17 D2S441 10,15 -
STR鑒定圖
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參考文獻(xiàn)
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D3S-001, a KRAS G12C inhibitor with rapid target engagement kinetics, overcomes nucleotide cycling and demonstrates robust preclinical and clinical activities(2024/05/08)
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Mitochondrial DNA content reduction induces aerobic glycolysis and reversible resistance to drug-induced apoptosis in SW480 colorectal cancer cells(2018/04/24)
作者：Jingjing Mou, Jin Peng, Yingying Shi
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DOI：10.1021/acs.jmedchem.3c00075
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期刊：Cancer Cell International
影響因子 ：5.800
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LncRNA-ENST00000543604 exerts a tumor-promoting effect via miRNA 564/AEG-1 or ZNF326/EMT and predicts the prognosis of and chemotherapeutic effect in colorectal cancer.(2022/08/23)
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影響因子 ：5.600
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Circ_0011385 knockdown inhibits cell proliferation, migration and invasion, whereas promotes cell apoptosis by regulating miR-330-3p/MYO6 axis in colorectal cancer(2022/01/26)
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期刊：Biomedical Journal
影響因子 ：5.500
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Raltitrexed as a synergistic hyperthermia chemotherapy drug screened in patient-derived colorectal cancer organoids(2021/8/15)
作者：Lisi Zeng, Quanxing Liao, Haoran Zhao
期刊：Cancer Biology & Medicine
DOI：10.20892/j.issn.2095-3941.2020.0566
影響因子 ：5.500
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m6A methyltransferase METTL16 mediates immune evasion of colorectal cancer cells via epigenetically regulating PD-L1 expression(2023/08/29)
作者：Ailei Wang, Yingjie Sun, Xince Wang
期刊：Aging-US
影響因子 ：5.200
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Lycorine Induces autophagy-associated apoptosis by targeting MEK2 and enhances vemurafenib activity in colorectal cancer(2020/01/03)
作者：Man Hu, Zhaomin Yu, Peiyuan Mei
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