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Calu-3 (人肺腺癌細(xì)胞(胸水)) (STR鑒定正確)
貨號(hào):CL-0054
規(guī)格:1×10?cells/T25培養(yǎng)瓶
價(jià)格: ¥1600
生長培養(yǎng)基:MEM(含NEAA)+10% FBS+1% P/S
產(chǎn)品概述
名稱 | Calu-3 (人肺腺癌細(xì)胞(胸水)) (STR鑒定正確) |
別稱 | CaLu-3; CALU-3; Calu 3; Calu3; CALU3 |
種屬 | 人 |
生長特性 | 貼壁細(xì)胞 |
細(xì)胞形態(tài) | 上皮細(xì)胞樣 |
凍存條件 | 凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度:液氮 |
培養(yǎng)方案A(默認(rèn)) |
生長培養(yǎng)基:
培養(yǎng)條件:
氣相:空氣,95%;CO2,5%, 溫度:37℃
|
推薦傳代比例 | 1:3-1:4 |
推薦換液頻率 | 2-3次/周 |
注意事項(xiàng) | 該細(xì)胞較難消化,注意延長消化時(shí)間,消化到細(xì)胞收縮變圓,輕拍培養(yǎng)瓶側(cè)邊,細(xì)胞可以滑落方可終止消化。 |
背景描述 | Calu-3細(xì)胞是從一名25歲的白人男性肺腺癌患者的胸水中分離建系的;該患者曾使用過環(huán)磷酰胺、博來霉素、阿霉素進(jìn)行治療。Calu-3細(xì)胞接種至裸鼠可成瘤;Calu-3細(xì)胞亦可作轉(zhuǎn)染宿主。 |
年齡(性別) | 男性;25歲 |
組織來源 | 肺;轉(zhuǎn)移灶;胸水腺癌 |
細(xì)胞類型 | 腫瘤細(xì)胞 |
腫瘤類型 | 肺癌細(xì)胞 |
生物安全等級(jí) | BSL-1 |
倍增時(shí)間 | ~35-45 hours |
致瘤性 | Yes, forms well differentiated grade I adenocarcinoma in nude mice. |
抗原表達(dá)情況 | Blood Type A; Rh+ |
基因表達(dá)情況 | Blood Type A; Rh+ |
保藏機(jī)構(gòu) | ATCC; HTB-55 BCRJ; 0264 |
STR鑒定
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STR位點(diǎn)信息
Amelogenin X CSF1PO 11,12 D2S1338 19 D3S1358 15,18 D5S818 11 D7S820 10,11 D8S1179 11,15 D13S317 12 D16S539 12,14 D18S51 14 ,17 D19S433 12,13 D21S11 28,30 FGA 25 PentaD 9,16 PentaE 5,21 TH01 6,9.3 TPOX 8 vWA 16,17 D6S1043 11 D12S391 21,22 D2S441 14 -
STR鑒定圖
參考文獻(xiàn)
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APOE interacts with ACE2 inhibiting SARS-CoV-2 cellular entry and inflammation in COVID-19 patients(2022/08/01)
作者:Hongsheng Zhang, Lin Shao, Zhihao Lin
期刊:Signal Transduction and Targeted Therapy
DOI:10.1038/s41392-022-01118-4
影響因子 :39.300
引用產(chǎn)品:CL-0054
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The HNF4α-BC200-FMR1 positive feedback loop promotes growth and metastasis in invasive mucinous lung adenocarcinoma(2021/01/01)
作者:Xiong Chen, Yujie Zhao, Daxuan Wang
期刊:Cancer Res?
DOI:10.1158/0008-5472.CAN-21-0980
影響因子 :11.200
引用產(chǎn)品:CL-0054
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Downregulation of the Circadian Rhythm Regulator HLF Promotes Multiple-Organ Distant Metastases in Non-Small Cell Lung Cancer Through PPAR NF-κb Signaling(2020/07/10)
作者:Aibin Liu
期刊:Cancer Letters
DOI:10.1016/j.canlet.2020.04.007
影響因子 :9.700
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Targeting TYK2 alleviates Rab27A-induced malignant progression of non-small cell lung cancer via disrupting IFNα-TYK2-STAT-HSPA5 axis(2024/03/23)
作者:Yuanyuan Zeng, Jian Zhao, Zhengyan Wu
期刊:npj Precision Oncology
DOI:10.1038/s41698-024-00574-1
影響因子 :7.900
引用產(chǎn)品:CL-0054
-
Activation of Hypoxia Inducible Factor-1 Alpha-Mediated DNA Methylation Enzymes (DNMT3a and TET2) Under Hypoxic Conditions Regulates S100A6 Transcription to Promote Lung Cancer Cell Growth and Metastasis(2024/03/18)
作者:Tengfei Wang, Genbao Zhu, Bo Wang
期刊:ANTIOXIDANTS & REDOX SIGNALING
影響因子 :6.600
引用產(chǎn)品:CL-0054
-
Characteristics of replication and pathogenicity of SARS-CoV-2 Alpha and Delta isolates(2022/09/24)
作者:Xiaoli Feng, Dandan Yu, Mi Zhang
期刊:VIROLOGICA SINICA
DOI:10.1016/j.virs.2022.09.007
影響因子 :5.500
引用產(chǎn)品:CL-0054
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GPR37-centered ceRNA network contributes to metastatic potential in lung adenocarcinoma: Evidence from high-throughput sequencing(2023/11/16)
作者:Chuanhui Chen, Mengzhi Wan, Xiong Peng
期刊:Translational Oncology
DOI:10.1016/j.tranon.2023.101819
影響因子 :5.000
引用產(chǎn)品:CL-0054
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Synergistic inhibition effects of andrographolide and baicalin on coronavirus mechanisms by downregulation of ACE2 protein level(2024/02/21)
作者:Lina Wan, Yuchen Li, Wenhao Liao
期刊:Scientific Reports
DOI:10.1038/s41598-024-54722-5
影響因子 :4.600
引用產(chǎn)品:CL-0054
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Decreased Sphingosine Due to Down-Regulation of Acid Ceramidase Expression in Airway of Bronchiectasis Patients: A Potential Contributor to Pseudomonas aeruginosa Infection(2023/04/27)
作者:Qian Qi, Jiawei Xu, Yujiao Wang
期刊:Infection and Drug Resistance
影響因子 :3.900
引用產(chǎn)品:CL-0054
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Comprehensive Analysis of a Novel Immune-Related Gene Signature in Lung Adenocarcinoma(2022/10/19)
作者:Hongxiang Feng, Chaoyang Liang, Yuhui Shi
期刊:Journal of Clinical Medicine
影響因子 :3.900
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CircRNA ZNF609 Knockdown Represses the Development of Non-Small Cell Lung Cancer via miR-623/FOXM1 Axis(2022/12/21)
作者:Fanghan Wang, Xiangfeng Li, Xigao Jia
期刊:Cancer Management and Research
影響因子 :3.602
引用產(chǎn)品:CL-0054
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Hsa_circ_0080608 Attenuates Lung Cancer Progression by Functioning as a Competitive Endogenous RNA to Regulate the miR-661/ADRA1A Pathway(2023/10/11)
作者:Chengbo Ren, Ling Cui, Ruibiao Li
期刊:HORMONE AND METABOLIC RESEARCH
影響因子 :2.200
引用產(chǎn)品:CL-0054
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