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產(chǎn)品概述
名稱 | SK-N-BE(2) (人神經(jīng)母細(xì)胞瘤細(xì)胞) (STR鑒定正確) |
別稱 | SK-N-BE2; SKNBE(2); SKNBE-2; SKNBE2; SK-N-BE; SKNBE |
種屬 | 人 |
生長(zhǎng)特性 | 半貼半懸 |
細(xì)胞形態(tài) | 神經(jīng)母細(xì)胞樣 |
凍存條件 | 凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度:液氮 |
培養(yǎng)方案A(默認(rèn)) |
培養(yǎng)條件:
氣相:空氣,95%;CO2,5%, 溫度:37℃
|
推薦傳代比例 | 1:3-1:4 |
推薦換液頻率 | 2-3次/周 |
背景描述 | SK-N-BE(2)神經(jīng)母細(xì)胞瘤細(xì)胞株是于1972年11月從一位多次化療及放療的擴(kuò)散性神經(jīng)母細(xì)胞瘤患兒骨髓穿刺物中建立的;SK-N-BE(2)細(xì)胞顯示中等水平的多巴胺-β-羥基酶活性。有報(bào)道稱,SK-N-BE(2)細(xì)胞的飽和濃度超過(guò)1×10^6細(xì)胞/cm^2。SK-N-BE(2)細(xì)胞形態(tài)多樣,有的有長(zhǎng)突觸、有的呈上皮細(xì)胞樣;SK-N-BE(2)細(xì)胞會(huì)聚集,形成團(tuán)塊并浮起。 |
年齡(性別) | 男性;2歲 |
組織來(lái)源 | 腦;神經(jīng)母細(xì)胞瘤,骨髓轉(zhuǎn)移灶 |
細(xì)胞類型 | 腫瘤細(xì)胞 |
腫瘤類型 | 神經(jīng)母細(xì)胞瘤細(xì)胞 |
生物安全等級(jí) | BSL-1 |
倍增時(shí)間 | ~36-48 hours |
致瘤性 | Yes, an inoculum of 1×10^7 cells produced tumors in cortisonized hamster cheek pouches with 18% frequency. |
保藏機(jī)構(gòu) | ATCC; CRL-2271 DSMZ; ACC-632 ECACC; 95011815 |
STR鑒定
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STR位點(diǎn)信息
Amelogenin X,Y CSF1PO 10 D2S1338 17,23 D3S1358 19 D5S818 12 D7S820 9,10 D8S1179 13,14 D13S317 11 D16S539 9,11 D18S51 16 D19S433 12,13 D21S11 30,32.2 FGA 22,25 PentaD 13,14 PentaE 14,18 TH01 6,7 TPOX 8,11 vWA 18 D6S1043 11,19 D12S391 18,24 D2S441 11,14 -
STR鑒定圖
參考文獻(xiàn)
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Enhancing the sensitization of neuroblastoma to radiotherapy by the construction of a dual-channel parallel free radicals nanoamplifier(2023/10/03)
作者:Wenxin Zhang, Xiaodie Li, Jialin Zeng
期刊:Materials Today Bio
DOI:10.1016/j.mtbio.2023.100828
影響因子 :8.200
引用產(chǎn)品:CL-0441
-
USP29 activation mediated by FUBP1 promotes AURKB stability and oncogenic functions in gastric cancer(2024/01/17)
作者:Rongfu Tu, Ye Kang, Yiwen Pan
期刊:Cancer Cell International
DOI:10.1186/s12935-024-03224-5
影響因子 :5.800
引用產(chǎn)品:CL-0441
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Mechanism of Endogenous Peptide PDYBX1 and Precursor Protein YBX1 in Hirschsprung’s Disease(2023/10/01)
作者:Qiaochu Sun, Zhengke Zhi, Chenglong Wang
期刊:Neuroscience Bulletin
DOI:10.1007/s12264-023-01132-8
影響因子 :5.600
引用產(chǎn)品:CL-0441
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HMGB3 is Associated With an Unfavorable Prognosis of Neuroblastoma and Promotes Tumor Progression by Mediating TPX2(2021/12/20)
作者:Xiaodan Zhong, Songling Zhang
期刊:Frontiers in Cell and Developmental Biology
影響因子 :5.500
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Silencing of long chain noncoding RNA paternally expressed gene (PEG10) inhibits the progression of neuroblastoma by regulating microRNA-449a (miR-449a)/ribosomal protein S2 (RPS2) axis(2022/02/25)
作者:Jian Zhang, Wei Liu, Ping Ji
期刊:Bioengineered
DOI:10.1080/21655979.2022.2042999
影響因子 :4.900
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Potential Cause-and-Effect Relationship between Gut Microbiota and Childhood Neuroblastoma: A Mendelian Randomization Analysis(2024/03/27)
作者:Jing Chu
期刊:INDIAN JOURNAL OF PEDIATRICS
DOI:10.1007/s12098-024-05065-6
影響因子 :4.300
引用產(chǎn)品:CL-0441
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