產(chǎn)品中心Cell Resources
聯(lián)系我們CONTACT US
400-999-210024小時(shí)服務(wù)熱線
產(chǎn)品概述
| 名稱 | NCI-H520 (人肺鱗癌細(xì)胞) (STR鑒定正確) |
| 別稱 | H520; H-520; NCI-HUT-520; NCIH520 |
| 種屬 | 人 |
| 生長(zhǎng)特性 | 貼壁細(xì)胞 |
| 細(xì)胞形態(tài) | 上皮細(xì)胞樣 |
| 凍存條件 | 凍存液：55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度：液氮 |
| 培養(yǎng)方案A（默認(rèn)） |
培養(yǎng)條件:
氣相：空氣，95%；CO2，5%, 溫度：37℃
|
| 推薦傳代比例 | 1:3-1:4 |
| 推薦換液頻率 | 2-3次/周 |
| 背景描述 | NCI-H520細(xì)胞是由Gazdar AF等在1982年建系而來(lái)，源于鱗狀細(xì)胞肺癌組織；NCI-H520細(xì)胞p53 mRNA表達(dá)水平較正常肺組織低，但是沒(méi)有大的結(jié)構(gòu)DNA的異常。NCI-H520細(xì)胞角蛋白、波形蛋白陽(yáng)性，神經(jīng)絲三聯(lián)蛋白陰性；NCI-H520細(xì)胞在軟瓊脂中可形成克隆。 |
| 組織來(lái)源 | 肺 |
| 細(xì)胞類型 | 腫瘤細(xì)胞 |
| 腫瘤類型 | 肺癌細(xì)胞 |
| 生物安全等級(jí) | BSL-1 |
| 倍增時(shí)間 | ~32-60 hours |
| 致瘤性 | Yes, in nude mice inoculated subcutaneously with 1×10^7 cells (Tumors developed within 21 days at 100% frequency (5/5)). |
| 保藏機(jī)構(gòu) | ATCC; HTB-182 |
STR鑒定
-
STR位點(diǎn)信息
Amelogenin X CSF1PO 10 D2S1338 18,23 D3S1358 16 D5S818 12,13 D7S820 8,12 D8S1179 14,16,17 D13S317 10,11 D16S539 13 D18S51 17 D19S433 13,14 D21S11 30 FGA 22 PentaD 13 PentaE 5,14 TH01 10 TPOX 8 vWA 18,19 D6S1043 12,18 D12S391 21 D2S441 10,11 -
STR鑒定圖
-
參考文獻(xiàn)
-
Single-cell transcriptomic sequencing data reveal aberrant DNA methylation in SMAD3 promoter region in tumor-associated fibroblasts affecting molecular mechanism of radiosensitivity in non-small cell lung cancer(2024/03/16)
作者：Fushi Han, Shuzhen Chen, Kangwei Zhang
期刊：Journal of Translational Medicine
DOI：10.1186/s12967-024-05057-2
影響因子 ：7.400
引用產(chǎn)品：CL-0402
-
Silencing ZIC2 abrogates tumorigenesis and anoikis resistance of non-small cell lung cancer cells by inhibiting Src/FAK signaling(2021/05/29)
作者：Aibin Liu, Huayan Xie, Ronggang Li
期刊：Molecular Therapy-Oncolytics
DOI：10.1016/j.omto.2021.05.008
影響因子 ：5.700
-
Tambulin Targets Histone Deacetylase 1 Inhibiting Cell Growth and Inducing Apoptosis in Human Lung Squamous Cell Carcinoma(2020/08/12)
作者：Wuming Wang, Yuzhen Liu, Long Zhao
期刊：Frontiers in Pharmacology
影響因子 ：5.600
-
Combined Inhibition of UBE2C and PLK1 Reduce Cell Proliferation and Arrest Cell Cycle by Affecting ACLY in Pan-Cancer(2023/10/27)
作者：Keying Liang, Qian Wang, Li Qiu
期刊：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
影響因子 ：5.600
引用產(chǎn)品：CL-0402
-
Celastrus orbiculatus Thunb. extract targeting DJ-1 inhibits non-small cell lung cancer invasion and metastasis through mitochondrial-induced ROS accumulation(2023/07/20)
作者：Xiaochen Ni, Shilong Yu, Xiaomin Jiang
期刊：JOURNAL OF ETHNOPHARMACOLOGY
影響因子 ：5.400
-
Development and validation of a N6 methylation regulator-related gene signature for prognostic and immune response prediction in non-small cell lung cancer(2023/07/15)
作者：Xiang Li, Jinlong Ma, Zhenqian Sun
期刊：American Journal of Cancer Research
DOI：PMID:37560004
影響因子 ：5.300
-
Tumor-infiltrating platelets promote the growth of lung adenocarcinoma(2023/10/27)
作者：Xiaohui Li, Ming Li, Ziming Hu
期刊：Translational Oncology
DOI：10.1016/j.tranon.2023.101813
影響因子 ：5.000
引用產(chǎn)品：CL-0402
-
Knockdown of lysine (K)-specific demethylase 2B KDM2B inhibits glycolysis and induces autophagy in lung squamous cell carcinoma cells by regulating the phosphatidylinositol 3-kinase/AKT/mammalian target of rapamycin pathway(2021/12/07)
作者：Zhonghai Xie, Hongwei Li, Jin Zang
期刊：Bioengineered
DOI：10.1080/21655979.2021.2005931
影響因子 ：4.900
-
Restraint of chaperonin containing T-complex protein-1 subunit 3 has antitumor roles in non-small cell lung cancer via affection of YAP1(2022/02/17)
作者：Hongyang Shi, Yonghong Zhang, Yu Wang
期刊：Toxicology And Applied Pharmacology
DOI：10.1016/j.taap.2022.115926
影響因子 ：4.460
-
Regulation of tumorigenesis and ferroptosis in non-small cell lung cancer by a novel BBOX1-AS1/miR-326/PROM2 axis(2023/08/28)
作者：Jinlu An, Jiang Shi, Chao Yang
期刊：MOLECULAR AND CELLULAR BIOCHEMISTRY
DOI：10.1007/s11010-023-04837-6
影響因子 ：4.300
引用產(chǎn)品：CL-0016 CL-0165 CL-0213 CL-0402 PM150110 PM150210 PM150410 PM150910
-
TMED3 exerts a protumor function in non-small cell lung cancer by enhancing the Wnt/β-catenin pathway via regulation of AKT(2021/11/7)
作者：Danjie Zhang, Liangzhang Sun, Jin Zhang
期刊：Toxicology And Applied Pharmacology
DOI：10.1016/j.taap.2021.115793
影響因子 ：3.800
-
SLC6A8 is involved in the progression of non-small cell lung cancer through the Notch signaling pathway(2020/10/27)
作者：Yan Feng, Xiangyu Guo, Huaping Tang
期刊：Annals of Translational Medicine
影響因子 ：3.616
-
Resveratrol improves the cytotoxic effect of CD8?+T cells in the tumor microenvironment by regulating HMMR/Ferroptosis in lung squamous cell carcinoma(2023/03/15)
作者：Shan Gao, Minchao Kang, Jizhao Wang
期刊：JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
DOI：10.1016/j.jpba.2023.115346
影響因子 ：3.400
引用產(chǎn)品：CL-0402
-
Hydrogen Peroxide-Inducible PROTACs for Targeted Protein Degradation in Cancer Cells(2023/07/18)
作者：Dehao Yu, Heli Fan, Zhili Zhou
期刊：CHEMBIOCHEM
影響因子 ：3.200
-
Calponin 3 suppresses proliferation, migration and invasion of non?small cell lung cancer cells(2021/7/1)
作者：Chenglin Yang, Shiping Zhu, Weifeng Feng
期刊：Oncology Letters
影響因子 ：2.900
-
Palbociclib Induces the Apoptosis of Lung Squamous Cell Carcinoma Cells via RB-Independent STAT3 Phosphorylation(2022/08/18)
作者：Wenjing Xiang, Wanchen Qi, Huayu Li
期刊：Current Oncology
影響因子 ：2.600
引用產(chǎn)品：CL-0402
-
Liquiritigenin Induces Cell Cycle Arrest and Apoptosis in Lung Squamous Cell Carcinoma(2024/05/22)
作者：Yaqi Liu, Yixiao Wang, Yiran Yang
期刊：CELL BIOCHEMISTRY AND BIOPHYSICS
DOI：10.1007/s12013-024-01294-w
影響因子 ：2.600
引用產(chǎn)品：CL-0402
FAQs
Q：{{item.question}}
A：
產(chǎn)品資料
識(shí)別碼示意圖
娜胡文君.png "邱國(guó)娜/胡文君")
.png "梅文強(qiáng)")
