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產品概述
名稱 | A549/DDP (人肺腺癌耐順鉑株)(STR鑒定正確) |
種屬 | 人 |
生長特性 | 貼壁細胞 |
細胞形態(tài) | 上皮細胞樣 |
凍存條件 | 凍存液:55% 基礎培養(yǎng)基+40%FBS+5%DMSO 溫度:液氮 |
培養(yǎng)方案A(默認) |
生長培養(yǎng)基:
培養(yǎng)條件:
氣相:空氣,95%;CO2,5%, 溫度:37℃
|
推薦傳代比例 | 1:2-1:3 |
推薦換液頻率 | 2-3次/周 |
注意事項 | 耐藥株生長速度慢,1:2傳代一周左右可以再次傳代,細胞形態(tài)比較梭長。當細胞生長速度過慢時,可以采用以下方式進行擴增培養(yǎng):用含低濃度藥物的培養(yǎng)基連續(xù)培養(yǎng)2周,期間根據(jù)細胞密度,需要傳代時,傳代處理。待細胞狀態(tài)恢復后,提高培養(yǎng)基中藥物的濃度進行培養(yǎng),此時梯度加藥,每個濃度培養(yǎng)一個代次(即持續(xù)培養(yǎng)到長滿傳代,即換下一個濃度),之后細胞狀態(tài)沒有問題的話,持續(xù)用加藥培養(yǎng)基進行培養(yǎng)即可。 |
細胞類型 | 腫瘤細胞 |
腫瘤類型 | 肺癌細胞 |
生物安全等級 | BSL-1 |
STR鑒定
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STR位點信息
Amelogenin X,Y CSF1PO 10,12 D2S1338 24 D3S1358 16 D5S818 11 D7S820 8,11 D8S1179 13,14 D13S317 11 D16S539 11,12 D18S51 14,17 D19S433 13 D21S11 29 FGA 23 PentaD 9 PentaE 7,11 TH01 8,9.3 TPOX 8,11 vWA 14 D6S1043 11,13 D12S391 18 D2S441 10,13 -
STR鑒定圖
參考文獻
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Hypoxia-triggered tumor specific glutamine inhibition for reversing cisplatin resistance of chemotherapy(2023/11/27)
作者:Jiaying Zhang, Jinghui Hu, Weiwei Zhu
期刊:CHEMICAL ENGINEERING JOURNAL
影響因子 :15.100
引用產品:CL-0519
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Inhibition of autophagy potentiates the cytotoxicity of the irreversible FGFR1-4 inhibitor FIIN-2 on lung adenocarcinoma(2022/08/30)
作者:Xiuqin Jia, Ming Xin, Juanjuan Xu
期刊:Cell Death & Disease
DOI:10.1038/s41419-022-05201-0
影響因子 :9.000
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Self-Assembly of Erlotinib-Platinum(II) Complexes for Epidermal Growth Factor Receptor-Targeted Photodynamic Therapy(2024/01/06)
作者:Haobing Wang, Yidan Lai, Dan Li
期刊:JOURNAL OF MEDICINAL CHEMISTRY
DOI:10.1021/acs.jmedchem.3c01889
影響因子 :7.300
引用產品:CL-0519
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A Clinically Translatable Ternary Platinum(IV) Prodrug for Synergistically Reversing Drug Resistance(2023/03/10)
作者:Ying Liu, Dun Wang, Hongbing Liu, Li Liu
期刊:JOURNAL OF MEDICINAL CHEMISTRY
DOI:10.1021/acs.jmedchem.2c01924
影響因子 :7.300
引用產品:CL-0519
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CXCR5 guides migration and tumor eradication of anti-EGFR chimeric antigen receptor T?cells(2021/07/16)
作者:Guangchao Li, Jintao Guo, Yanfang Zheng
期刊:Molecular Therapy-Oncolytics
DOI:10.1016/j.omto.2021.07.003
影響因子 :5.700
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Targeting Polo-like Kinase 1 by a Novel Pyrrole-Imidazole Polyamide–Hoechst Conjugate Suppresses Tumor Growth In Vivo(2018/05/01)
作者:Ke Liu, Lijing Fang, Haiyan Sun
期刊:Molecular Cancer Therapeutics
DOI:10.1158/1535-7163.MCT-17-0747
影響因子 :5.700
-
Assessment of Mononuclear/Dinuclear copper acylhydrazone complexes for lung cancer treatment(2024/01/14)
作者:Youru Wu, Daqi Wu, Jianfeng Lan
期刊:BIOORGANIC CHEMISTRY
DOI:10.1016/j.bioorg.2024.107122
影響因子 :5.100
引用產品:CL-0519
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A ratiometric near-infrared fluorescence strategy based on spiropyran in situ switching for tracking dynamic changes of live-cell lysosomal pH(2019/03/27)
作者:Jin Li, Xiaokang Li, Jianbo Jia
期刊:Dyes And Pigments
DOI:10.1016/j.dyepig.2019.03.060
影響因子 :4.500
-
A novel colorimetric and ratiometric fluorescent probe for selective detection of bisulfite in real samples and living cells(2018/12/07)
作者:Jin Li, Ying Gao, Haoran Guo
期刊:Dyes And Pigments
DOI:10.1016/j.dyepig.2018.12.009
影響因子 :4.500
引用產品:CL-0519
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A cisplatin and disulfiram co-loaded inclusion complex overcomes drug resistance by inhibiting cancer cell stemness in non-small cell lung cancer(2023/12/22)
作者:Wenhui Ye, Huaiyou Lv, Qinxiu Zhang
期刊:JOURNAL OF DRUG TARGETING
DOI:10.1080/1061186X.2023.2298844
影響因子 :4.500
引用產品:CL-0519
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Circ_0006988 promotes the proliferation, metastasis and angiogenesis of non-small cell lung cancer cells by modulating miR-491-5p/MAP3K3 axis(2021/06/30)
作者:Chao Yang, Jiang Shi, Jie Wang
期刊:Cell Cycle
DOI:10.1080/15384101.2021.1941612
影響因子 :4.300
-
Circ_0017956 promotes the proliferation and metastasis of non-small cell lung cancer through regulating miR-515-5p/ITGB8 axis(2021/05/04)
作者:Na Li, Dan Zhu
期刊:Cell Cycle
DOI:10.1080/15384101.2021.1919829
影響因子 :4.300
-
Mixed-ligand copper(II) hydrazone complexes: Synthesis, structure, and anti-lung cancer properties(2023/01/19)
作者:YouRu Wu, LiXia Hou, JianFeng Lan
期刊:JOURNAL OF MOLECULAR STRUCTURE
DOI:10.1016/j.molstruc.2023.134986
影響因子 :3.800
-
p32/OPA1 axis-mediated mitochondrial dynamics contributes to cisplatin resistance in non-small cell lung cancer: p32/OPA1 contributes to cisplatin resistance(2023/12/26)
作者:Chunxia Yu, Zheqing Peng, Tao Wang
期刊:ACTA BIOCHIMICA ET BIOPHYSICA SINICA
影響因子 :3.700
引用產品:CL-0519
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Role and mechanism of COL3A1 in regulating the growth, metastasis, and drug sensitivity in cisplatin-resistant non-small cell lung cancer cells(2024/03/26)
作者:Jiankun Ren, Songwei Zhao, Junyu Lai
期刊:CANCER BIOLOGY & THERAPY
DOI:10.1080/15384047.2024.2328382
影響因子 :3.600
引用產品:CL-0519
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Mechanism of PWAR6 regulating cisplatin drug sensitivity in non-small cell lung cancer through miR-577/PHACTR1(2023/11/03)
作者:Wenjun Tang, Jingru Luo, Shu Lin
期刊:GENE
DOI:10.1016/j.gene.2023.147954
影響因子 :3.500
引用產品:CL-0519
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Demethoxycurcumin increases the sensitivity of cisplatin?resistant non?small lung cancer cells to cisplatin and induces apoptosis by activating the caspase signaling pathway(2020/9/8)
作者:Yun Chen, Chaojin Hong, Xiaochen Chen
期刊:Oncology Letters
影響因子 :2.900
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Overexpression of close homolog of L1 enhances the chemosensitivity of lung cancer cells via inhibition of the Akt pathway(2020/08/12)
作者:Xiangdao Cai, Bang Hu, Sheng Liu
期刊:Oncology Letters
影響因子 :2.900
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Andrographis modulates cisplatin resistance in lung cancer via miR-155-5p/SIRT1 axis(2023/08/02)
作者:Chong Pang, Tengyue Zhang, Yulong Chen
期刊:FUNCTIONAL & INTEGRATIVE GENOMICS
DOI:10.1007/s10142-023-01186-x
影響因子 :2.900
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The impact of SOX4-activated CTHRC1 transcriptional activity regulating DNA damage repair on cisplatin resistance in lung adenocarcinoma(2024/04/17)
作者:Cheng Ai, Zhenhao Huang, Tenghao Rong
期刊:ELECTROPHORESIS
影響因子 :2.900
引用產品:CL-0519
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Anlotinib Inhibits Cisplatin Resistance in Non-Small-Cell Lung Cancer Cells by Inhibiting MCL-1 Expression via MET/STAT3/Akt Pathway(2024/03/04)
作者:Lile Wang, Lu Xu, Shuhua Han
期刊:Canadian Respiratory Journal
影響因子 :2.200
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MicroRNA-106a-5p alleviated the resistance of cisplatin in lung cancer cells by targeting Jumonji domain containing 6(2021/10/02)
作者:Xiang Ge,?Yifei Jiang,?Xun Hu
期刊:Transplant Immunology
DOI:10.1016/j.trim.2021.101478
影響因子 :2.032
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