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產(chǎn)品概述
名稱 | LNCaP clone FGC (人前列腺癌細胞) (STR鑒定正確) |
別稱 | LNCaP-Clone-FGC; LNCaP.FGC; LNCaP-FGC; LNCaP FGC; LNCaP-ATCC |
種屬 | 人 |
生長特性 | 貼壁細胞 |
細胞形態(tài) | 上皮細胞樣 |
凍存條件 | 凍存液:55% 基礎培養(yǎng)基+40%FBS+5%DMSO 溫度:液氮 |
培養(yǎng)方案A(默認) |
培養(yǎng)條件:
氣相:空氣,95%;CO2,5%, 溫度:37℃
|
推薦傳代比例 | 1:3-1:4 |
推薦換液頻率 | 2-3次/周 |
注意事項 | 1.該細胞并不形成一致的單層,而是形成集落。2.該細胞會使培養(yǎng)基快速變酸,且生長緩慢,故傳代后 48 小時內(nèi)不應擾動;3.普通TC處理的培養(yǎng)瓶或皿不能使細胞很好的貼壁,培養(yǎng)難度較高,需使用 Corning 的 cellbind 細胞培養(yǎng)瓶(貨號是 3289),或者使用多聚-L-賴氨酸溶液(貨號 PB180523)包被過的培養(yǎng)皿;4.在細胞運輸途中,多數(shù)細胞會從培養(yǎng)瓶底分離,大片懸浮在培養(yǎng)基中,按照收貨注意事項處理即可恢復正常。 |
背景描述 | 人前列腺癌細胞LNCaP clone FGC是從一位50歲白人男性(血型B+)的左鎖骨淋巴結(jié)針刺活檢中分離,該患者經(jīng)確診為前列腺癌轉(zhuǎn)移。LNCaP clone FGC細胞對5-α-二氫睪酮(生長調(diào)節(jié)子和酸性磷酸脂酶產(chǎn)物)有響應。LNCaP clone FGC細胞并不形成一致的單層,而是形成集落,在傳代時可以用滴管反復吹吸打碎。LNCaP clone FGC細胞僅僅輕輕地吸附在基底上,不形成匯合,很快使培養(yǎng)基變酸。LNCaP clone FGC細胞生長極其緩慢,傳代后48小時內(nèi)不應擾動。當培養(yǎng)瓶封包后,多數(shù)LNCaP clone FGC細胞從培養(yǎng)瓶底分離,懸浮在培養(yǎng)基中。收到后,在通常培養(yǎng)單層細胞的條件下培養(yǎng)24-48小時,以使細胞再貼壁。此后,可以換上新鮮培養(yǎng)液。如果需要,培養(yǎng)瓶內(nèi)容物可以收集,300g離心15分鐘,以10毫升培養(yǎng)液重懸并培養(yǎng)到一個單獨的培養(yǎng)瓶中。 |
年齡(性別) | 男性;50歲 |
組織來源 | 前列腺;左鎖骨淋巴結(jié)癌轉(zhuǎn)移灶 |
細胞類型 | 腫瘤細胞 |
腫瘤類型 | 前列腺癌細胞 |
生物安全等級 | BSL-1 |
倍增時間 | ~32-36 hours |
致瘤性 | Yes, in soft agar.Yes, the cells are tumorigenic in nude mice. |
受體表達情況 | androgen receptor, positive;estrogen receptor, positive |
基因表達情況 | human prostatic acid phosphatase; prostate specific antigen |
保藏機構(gòu) | ATCC; CRL-1740 BCRC; 60088 BCRJ; 0149 ECACC; 89110211 |
STR鑒定
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STR位點信息
Amelogenin X,Y CSF1PO 10,11 D2S1338 16 D3S1358 16 D5S818 11,12 D7S820 9.1,10.3 D8S1179 12,13,14 D13S317 10,12 D16S539 11 D18S51 11,12 D19S433 13.2,15 D21S11 29,32.2 FGA 19,20 PentaD 12,12.4 PentaE 12,16 TH01 9 TPOX 8,9 vWA 16,18 D6S1043 11,12,18 D12S391 21,22 D2S441 11.3 -
STR鑒定圖
參考文獻
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Integrated analysis reveals FOXA1 and Ku70/Ku80 as targets of ivermectin in prostate cancer(2022/09/01)
作者:Shidong Lv, Zeyu Wu, Mayao Luo
期刊:Cell Death & Disease
DOI:10.1038/s41419-022-05182-0
影響因子 :9.000
-
Galaxolide and Irgacure 369 are novel environmental androgens(2023/03/09)
作者:Mingzhao Li, Ren Wang, Pan Wang
期刊:CHEMOSPHERE
DOI:10.1016/j.chemosphere.2023.138329
影響因子 :8.800
-
ATXN3 promotes prostate cancer progression by stabilizing YAP(2023/06/22)
作者:Longxiang Wu, Zhenyu Ou, Peihua Liu
期刊:Cell Communication and Signaling
DOI:10.1186/s12964-023-01073-9
影響因子 :8.400
-
PEG-coated and Gd-loaded fluorescent silica nanoparticles for targeted prostate cancer magnetic resonance imaging and fluorescence imaging(2023/01/02)
作者:Wei Jiang, Huiying Fang, Fengqiu Liu
期刊:International Journal of Nanomedicine
影響因子 :8.000
引用產(chǎn)品:CL-0143
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ND630 controls ACACA and lipid reprogramming in prostate cancer by regulating the expression of circKIF18B_003(2023/12/04)
作者:Yupeng Wu, Wencai Zheng, Qi Huang
期刊:Journal of Translational Medicine
DOI:10.1186/s12967-023-04760-w
影響因子 :7.400
引用產(chǎn)品:CL-0143
-
Tautomycin and enzalutamide combination yields synergistic effects on castration-resistant prostate cancer(2022/11/29)
作者:Luo, Mayao, Zhang, Yifan, Xu, Zhuofan, Wu, Chenwei, Ye, Yuedian, Liu, Rui, Lv, Shidong, Wei, Qiang
期刊:Cell Death Discovery
DOI:10.1038/s41420-022-01257-1
影響因子 :7.000
-
Triggering pyroptosis enhances the antitumor efficacy of PARP inhibitors in prostate cancer(2023/08/23)
作者:Ao Tian, Tingyu Wu, Yanshuang Zhang
期刊:CELLULAR ONCOLOGY
DOI:10.1007/s13402-023-00860-3
影響因子 :6.600
-
β-hydroxybutyrate inhibits malignant phenotypes of prostate cancer cells through β-hydroxybutyrylation of indoleacetamide-N-methyltransferase(2024/03/30)
作者:Yifan Zhang, Yunlong Li
期刊:Cancer Cell International
DOI:10.1186/s12935-024-03277-6
影響因子 :5.800
引用產(chǎn)品:CL-0143
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AID-induced CXCL12 upregulation enhances castration-resistant prostate cancer cell metastasis by stabilizing β-catenin expression(2023/11/23)
作者:Qi Li, Jinfeng Fan, Zhiyan Zhou
期刊:iScience
DOI:10.1016/j.isci.2023.108523
影響因子 :5.800
引用產(chǎn)品:CL-0143
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LOC100996425 acts as a promoter in prostate cancer by mediating hepatocyte nuclear factor 4A and the AMPK/mTOR pathway(2021/08/19)
作者:Xiuyan Wang, Bin Song, Mingcui Zang
期刊:Journal Of Cellular And Molecular Medicine
影響因子 :5.300
-
MET inhibition enhances PARP inhibitor efficacy in castration-resistant prostate cancer by suppressing the ATM/ATR and PI3K/AKT pathways(2021/11/10)
作者:Sihai Zhou,?Zhihong Dai,?Liang Wang
期刊:Journal Of Cellular And Molecular Medicine
影響因子 :5.295
-
Cuproptosis enhances docetaxel chemosensitivity by inhibiting autophagy via the DLAT/mTOR pathway in prostate cancer(2023/08/16)
作者:Hongzhuang Wen, Changbao Qu, Zhu Wang
期刊:FASEB JOURNAL
影響因子 :4.800
-
Comprehensiveness cuproptosis related genes study for prognosis and medication sensitiveness across cancers, and validation in prostate cancer(2024/04/26)
作者:Longfei Yang, Yifan Tang, Yuwei Zhang
期刊:Scientific Reports
DOI:10.1038/s41598-024-57303-8
影響因子 :4.600
引用產(chǎn)品:CL-0143
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HMGB1 promotes the development of castration?resistant prostate cancer by regulating androgen receptor activation(2022/09/19)
作者:Junyu Chen, Duo Xu, Taiwei Wang
期刊:ONCOLOGY REPORTS
影響因子 :4.200
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PLA inhibits TNF-α-induced PANoptosis of prostate cancer cells through metabolic reprogramming(2024/02/24)
作者:Yinghui Hao, Fangmei Xie, Jieyi He
期刊:INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
DOI:10.1016/j.biocel.2024.106554
影響因子 :4.000
引用產(chǎn)品:CL-0143
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Analyzing roles of small nucleolar RNA host gene 25 from clinical, molecular target and tumor formation in prostate cancer(2023/06/10)
作者:Zelin Liu, Shuai Ke, Qinghua Wang
期刊:EXPERIMENTAL CELL RESEARCH
DOI:10.1016/j.yexcr.2023.113686
影響因子 :3.700
-
A cuproptosis-related LncRNA signature: Integrated analysis associated with biochemical recurrence and immune landscape in prostate cancer(2023/02/24)
作者:Lei Ren, Xu Yang, Weifeng Wang
期刊:Frontiers in Genetics
DOI:10.3389/fgene.2023.1096783
影響因子 :3.700
-
A Prognostic Model for Prostate Cancer Patients Based on Two DNA Damage Response Mutation-Related Immune Genes(2023/08/22)
作者:Jian Wang, Li Jiang, Zhenhua Shang
期刊:CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS
影響因子 :3.400
-
IU1 and enzalutamide combination yields synergistic effects on castration-resistant prostate cancer(2023/08/07)
作者:Yifan Zhang, Yuanpeng Liao, Mayao Luo
期刊:PROSTATE
影響因子 :2.800
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GPX2 predicts recurrence-free survival and triggers the Wnt/β-catenin/EMT pathway in prostate cancer(2022/10/25)
作者:Ming Yang, Xudong Zhu, Yang Shen
期刊:PeerJ
影響因子 :2.700
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Exploring the role of differentially expressed metabolic genes and their mechanisms in bone metastatic prostate cancer(2023/04/12)
作者:Qingfu Zhang, Peng Zhang, Zhongting Zhao
期刊:PeerJ
影響因子 :2.700
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Circ_0044516 Enriches the Level of SARM1 as a miR-330-5p Sponge to Regulate Cell Malignant Behaviors and Tumorigenesis of Prostate Cancer(2022/01/07)
作者:Yan Wu
期刊:Biochemical Genetics
DOI:10.1007/s10528-021-10160-w
影響因子 :2.400
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A morpholine derivative N-(4-morpholinomethylene)ethanesulfonamide induces ferroptosis in tumor cells by targeting NRF2(2024/01/30)
作者:Bingchun Sun, Ligang Zhang, Binhua Wu
期刊:BIOLOGICAL & PHARMACEUTICAL BULLETIN
影響因子 :2.000
引用產(chǎn)品:CL-0143
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